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ppcrscript amp sk(1) vector  (Agilent technologies)


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    Structured Review

    Agilent technologies ppcrscript amp sk(1) vector
    Ppcrscript Amp Sk(1) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript+amp+sk(1)+vector/pm12071705-53-1-12
    Average 90 stars, based on 1 article reviews
    ppcrscript amp sk(1) vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Expression, refolding, purification, molecular characterization, crystallization, and preliminary X-ray analysis of the receptor binding domain of human B7-2.
    Article Snippet: The cell-mediated immune response involves a series of specific molecular interactions between cell surface molecules on T cells and antigen-presenting cells.. Of particular importance for the regulation of T cell activity is the interaction of the B7 isoforms, B7-1 and B7-2, with the T cell surface costimulatory receptors, CD28 and CTLA-4.. The binding of CD28 by B7-1/B7-2 results in an enhancement of T cell responses initiated by the interaction between a clonotypic T cell receptor and its specific, antigenic MHC–peptide complex, whereas the subsequent engagement of CTLA-4 by B7-1/B7-2 leads to a down-regulation of the response.

    Article Title: A dominant negative CREB (cAMP response element-binding protein) isoform inhibits thyrocyte growth, thyroid-specific gene expression, differentiation, and function.
    Article Snippet: For in vitro transcription to generate RNA probes, a transgene-specific 300-bp fragment of the 39-untranslated region (39-UTR) of the Ser119Ala CREB mutant was cloned into the BamHI/EcoRI sites of the pPCRScript Amp SK (1) vector (Stratagene).

    Article Title: Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats.
    Article Snippet: This fragment shared no homology with any other proteins in the rat brain and was subcloned into the SrfI cloning site of pPCRScript Amp SK(1) cloning vector (Stratagene) to produce a template for riboprobe synthesis.

    Mutagenesis:

    Article Title: Expression, refolding, purification, molecular characterization, crystallization, and preliminary X-ray analysis of the receptor binding domain of human B7-2.
    Article Snippet: The cell-mediated immune response involves a series of specific molecular interactions between cell surface molecules on T cells and antigen-presenting cells.. Of particular importance for the regulation of T cell activity is the interaction of the B7 isoforms, B7-1 and B7-2, with the T cell surface costimulatory receptors, CD28 and CTLA-4.. The binding of CD28 by B7-1/B7-2 results in an enhancement of T cell responses initiated by the interaction between a clonotypic T cell receptor and its specific, antigenic MHC–peptide complex, whereas the subsequent engagement of CTLA-4 by B7-1/B7-2 leads to a down-regulation of the response.

    Article Title: A dominant negative CREB (cAMP response element-binding protein) isoform inhibits thyrocyte growth, thyroid-specific gene expression, differentiation, and function.
    Article Snippet: For in vitro transcription to generate RNA probes, a transgene-specific 300-bp fragment of the 39-untranslated region (39-UTR) of the Ser119Ala CREB mutant was cloned into the BamHI/EcoRI sites of the pPCRScript Amp SK (1) vector (Stratagene).

    Article Title: Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats.
    Article Snippet: This fragment shared no homology with any other proteins in the rat brain and was subcloned into the SrfI cloning site of pPCRScript Amp SK(1) cloning vector (Stratagene) to produce a template for riboprobe synthesis.

    Clone Assay:

    Article Title: Expression, refolding, purification, molecular characterization, crystallization, and preliminary X-ray analysis of the receptor binding domain of human B7-2.
    Article Snippet: The cell-mediated immune response involves a series of specific molecular interactions between cell surface molecules on T cells and antigen-presenting cells.. Of particular importance for the regulation of T cell activity is the interaction of the B7 isoforms, B7-1 and B7-2, with the T cell surface costimulatory receptors, CD28 and CTLA-4.. The binding of CD28 by B7-1/B7-2 results in an enhancement of T cell responses initiated by the interaction between a clonotypic T cell receptor and its specific, antigenic MHC–peptide complex, whereas the subsequent engagement of CTLA-4 by B7-1/B7-2 leads to a down-regulation of the response.

    Article Title: A dominant negative CREB (cAMP response element-binding protein) isoform inhibits thyrocyte growth, thyroid-specific gene expression, differentiation, and function.
    Article Snippet: For in vitro transcription to generate RNA probes, a transgene-specific 300-bp fragment of the 39-untranslated region (39-UTR) of the Ser119Ala CREB mutant was cloned into the BamHI/EcoRI sites of the pPCRScript Amp SK (1) vector (Stratagene).

    Article Title: Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats.
    Article Snippet: This fragment shared no homology with any other proteins in the rat brain and was subcloned into the SrfI cloning site of pPCRScript Amp SK(1) cloning vector (Stratagene) to produce a template for riboprobe synthesis.

    Plasmid Preparation:

    Article Title: Expression, refolding, purification, molecular characterization, crystallization, and preliminary X-ray analysis of the receptor binding domain of human B7-2.
    Article Snippet: The cell-mediated immune response involves a series of specific molecular interactions between cell surface molecules on T cells and antigen-presenting cells.. Of particular importance for the regulation of T cell activity is the interaction of the B7 isoforms, B7-1 and B7-2, with the T cell surface costimulatory receptors, CD28 and CTLA-4.. The binding of CD28 by B7-1/B7-2 results in an enhancement of T cell responses initiated by the interaction between a clonotypic T cell receptor and its specific, antigenic MHC–peptide complex, whereas the subsequent engagement of CTLA-4 by B7-1/B7-2 leads to a down-regulation of the response.

    Article Title: A dominant negative CREB (cAMP response element-binding protein) isoform inhibits thyrocyte growth, thyroid-specific gene expression, differentiation, and function.
    Article Snippet: For in vitro transcription to generate RNA probes, a transgene-specific 300-bp fragment of the 39-untranslated region (39-UTR) of the Ser119Ala CREB mutant was cloned into the BamHI/EcoRI sites of the pPCRScript Amp SK (1) vector (Stratagene).

    Article Title: Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats.
    Article Snippet: This fragment shared no homology with any other proteins in the rat brain and was subcloned into the SrfI cloning site of pPCRScript Amp SK(1) cloning vector (Stratagene) to produce a template for riboprobe synthesis.



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